Effects of transgenic corn and Cry1Ab protein on the nematode, Caenorhabditis elegans ☆

摘要: Abstract The effects of the insecticidal Cry1Ab protein from Bacillus thuringiensis ( Bt ) on nematode, Caenorhabditis elegans , were studied with soil experimental fields cultivated transgenic corn (MON810) and trypsinized expressed in Escherichia coli . content was above detection limit an ELISA test only half samples obtained plots, ranging 0.19 to 1.31 ng g −1 dry weight. In a laboratory bioassay, C. exposed rhizosphere bulk isogenic or solutions (0, 24, 41, 63, 118, 200 mg l over period 96 h, growth reproduction serving as parameters. Nematode significantly reduced compared correlated concentrations samples. Moreover, toxicity pure concentration-dependent. As significant inhibition occurred at relatively high (41 mg l ), could not be assigned directly protein. results demonstrate that bioassays provide promising tool for monitoring potential toxins aqueous medium soils.