Inter- and intra-laboratory standardization of TUNEL assay for assessment of sperm DNA fragmentation

作者: S. Ribeiro , R. Sharma , S. Gupta , Z. Cakar , C. De Geyter

DOI: 10.1111/ANDR.12334

关键词:

摘要: … The goal of this study was optimize the TUNEL protocol for … This inter- and intra-laboratory study establishes that TUNEL … for TUNEL validate the assay and establishes TUNEL as a …

参考文章(52)
Monica Muratori, Lara Tamburrino, Sara Marchiani, Marta Cambi, Biagio Olivito, Chiara Azzari, Gianni Forti, Elisabetta Baldi, Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress Molecular Medicine. ,vol. 21, pp. 109- 122 ,(2015) , 10.2119/MOLMED.2014.00158
José Luis Fernández, Dioleyda Cajigal, Carmen López-Fernández, Jaime Gosálvez, Assessing Sperm DNA Fragmentation with the Sperm Chromatin Dispersion Test Methods of Molecular Biology. ,vol. 682, pp. 291- 301 ,(2011) , 10.1007/978-1-60327-409-8_21
Monica Muratori, Sara Marchiani, Mario Maggi, Gianni Forti, Elisabetta Baldi, None, Origin and biological significance of DNA fragmentation in human spermatozoa Frontiers in Bioscience. ,vol. 11, pp. 1491- 1499 ,(2006) , 10.2741/1898
M. Sergerie, G. Laforest, L. Bujan, F. Bissonnette, G. Bleau, Sperm DNA fragmentation: threshold value in male fertility. Human Reproduction. ,vol. 20, pp. 3446- 3451 ,(2005) , 10.1093/HUMREP/DEI231
Martin Sergerie, Roger Mieusset, Françoise Croute, Myriam Daudin, Louis Bujan, High risk of temporary alteration of semen parameters after recent acute febrile illness. Fertility and Sterility. ,vol. 88, ,(2007) , 10.1016/J.FERTNSTERT.2006.12.045
Ralf Henkel, Eva Kierspel, Marjam Hajimohammad, Thomas Stalf, Christiaan Hoogendijk, Claas Mehnert, Roelof Menkveld, Wolf-Bernhard Schill, Thinus F Kruger, DNA fragmentation of spermatozoa and assisted reproduction technology. Reproductive Biomedicine Online. ,vol. 7, pp. 477- 484 ,(2003) , 10.1016/S1472-6483(10)61893-7