Myelogenous production and maturation of B lymphocytes in the mouse.

摘要: Cells of the B lymphocyte lineage in young adult murine bone marrow were identified and resolved into compartments based on cell size expression mu heavy chain IgM cytoplasm (cmu) or surface (smu). The proliferative status, renewal rate, intercompartmental transit cells through defined determined using established protocols vivo tritiated thymidine (3H-TdR) administration, followed by radioautography smears. In addition, we specifically tested whether any derived from long-lived lymphocytes that are known to enter marrow. Only large immediately incorporated DNA precursor both small cmu+ smu- smu+ postmitotic lymphocytes. Large found be a rapid compartment which last mitosis differentiation takes place. All divided only once, daughter entered population. relied for their maintenance entirely input an Ig- progenitor compartment. Progenitors not lymphocytes, proliferated less rapidly than descendants, maintained up 40 hr. Approximately 70% immediate division products cells, whereas remainder with no detectable synthesis. Small maturation cells. This conversion appeared take place at random rather as function age. Renewal times without smu calculated 48 96 hr, respectively. cmu- included other mu+ populations either replaced newly produced A conspicuous minor population detected, data relating these subjected several interpretations. These observations provide insight origin fate precursors suggest scheme terminal stages formation is similar myelogenous production hemopoietic