Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors.

摘要: Silicon is a promising material for tissue engineering since it allows to produce micropatterned scaffolding structures resembling biological tissues. Using specific fabrication methods, possible build aligned 3D network-like structures. In the present study, we exploited vertically-aligned silicon micropillar arrays as culture systems human iPSC-derived cortical progenitors. particular, our aim was mimic radially-oriented radial glia fibres that during embryonic development play key roles in controlling expansion, migration and differentiation of progenitors, which are, turn, pivotal establishment correct multilayered cerebral cortex structure. Here show vertical efficiently promote expansion stemness preservation progenitors when compared standard monolayer growth conditions. Furthermore, vertically-oriented micropillars allow distinctive vivo. These results indicate can offer an optimal system progenitors’ migration. similar attractive platform engineering.