Ganglioside isomer analysis using ion polarity switching liquid chromatography-tandem mass spectrometry.

摘要: Gangliosides are ubiquitously present on cell surface. They more abundantly expressed in nerve cells and tissues involved pathology of various diseases. Diversity molecular structures the carbohydrate head group, fatty acyl, long chain base increases complexity analyzing gangliosides. In this study, an ultrahigh-performance liquid chromatography-tandem mass spectrometry method is developed for analysis co-eluting ganglioside isomers, which uses ion polarity switching to integrate glycan isomer identification, ceramide differentiation, quantification into one analysis. The facilitated with extensive target list by combining groups, bases, experimentally determined acyls. Correlation between retention time its total carbon number validated used predict scheduling final multiple reaction monitoring method. This was according FDA guidelines: 96.5% gangliosides good accuracy (80–120%), precision (  0.99. authenticated were quantified from mouse brain isotope dilution. Overall, 165 using 10 mg tissue, including 100 isomers GM1, GM2, GM3, GD1a, GD1b, GD2, GD3, GT1b.