Endothelial Induced EMT in Breast Epithelial Cells with Stem Cell Properties
作者： Valgardur Sigurdsson , Bylgja Hilmarsdottir , Hekla Sigmundsdottir , Agla J. R. Fridriksdottir , Markus Ringnér
DOI: 10.1371/JOURNAL.PONE.0023833
关键词:
摘要: Epithelial to mesenchymal transition (EMT) is a critical event in cancer progression and closely linked the breast epithelial stem cell phenotype. Given close interaction between vascular endothelium cells, especially at invasive front, we asked whether endothelial cells might play role EMT. Using 3D culture model demonstrate that are potent inducers of EMT D492 an immortalized line with properties. Endothelial induced mesenchymal-like (D492M) derived from D492, show reduced expression keratins, switch E-Cadherin (E-Cad) N-Cadherin (N-Cad) enhanced migration. Acquisition associated characteristics like increased CD44(high)/CD24(low) ratio, resistance apoptosis anchorage independent growth was also seen D492M cells. partially blocked by inhibition HGF signaling. Basal-like cancer, rich properties adverse prognosis has been We immunostained several basal-like samples for markers. Cancer areas no or decreased E-Cad N-Cad suggesting Collectively, have shown Furthermore, contains phenotype, most prominently these tumors. conclude may cancer.
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