Low-level genomic instability is a feature of papillary thyroid carcinoma: an array comparative genomic hybridization study of laser capture microdissected papillary thyroid carcinoma tumors and clonal cell lines.
作者： Orla Sheils , Esther O'Regan , Mary Toner , John O'Leary , Stephen Finn
DOI: 10.1043/1543-2165(2007)131[65:LGIIAF]2.0.CO;2
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摘要: Abstract Context.—Previous chromosomal comparative genomic hybridization (CGH) studies of papillary thyroid carcinoma (PTC) have demonstrated a low prevalence aberrations, with the majority tumors showing no evidence instability. The technique CGH can be optimized, however, using array and laser capture microdissection to ensure pure cell populations for analysis. Objective.—To assess PTC applied microdissected tumor cells cultures. Design.—Well-characterized (known ret/PTC BRAF mutation status), including samples from 5 classic morphology, 3 follicular variant tumors, clonal lines, were analyzed. Results.—Copy gain loss occurred in all cases lines examined. most common recurrent aberrations involved gains on chromosomes 1, 5, 7, 11, 15, 17, 22, deletions occurring 4, 18, 19. Analysis data 8 showed th...
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