High pressure homogenization is a key unit operation in inclusion body processing

作者: Britta Eggenreich , David Johannes Wurm , Vignesh Rajamanickam , Robert Klausser , Christoph Slouka

DOI: 10.1016/J.BTECX.2020.100022

关键词:

摘要: … coli often leads to the formation of inclusion bodies (IBs). … of the refolded protein are the responses to evaluate an IB process. The objective of this case study was to develop a refolding …

参考文章(38)
Špela Peternel, Radovan Komel, Active Protein Aggregates Produced in Escherichia coli International Journal of Molecular Sciences. ,vol. 12, pp. 8275- 8287 ,(2011) , 10.3390/IJMS12118275
D.H.G. Pelegrine, C.A. Gasparetto, Whey proteins solubility as function of temperature and pH Lwt - Food Science and Technology. ,vol. 38, pp. 77- 80 ,(2005) , 10.1016/J.LWT.2004.03.013
M.M Carrió, A Villaverde, Construction and deconstruction of bacterial inclusion bodies. Journal of Biotechnology. ,vol. 96, pp. 3- 12 ,(2002) , 10.1016/S0168-1656(02)00032-9
Ana Ramón, Mario Señorale-Pose, Mónica Marín, Inclusion bodies: not that bad…. Frontiers in Microbiology. ,vol. 5, pp. 56- 56 ,(2014) , 10.3389/FMICB.2014.00056
Cornelia Walther, Sabrina Mayer, Alois Jungbauer, Astrid Dürauer, Getting ready for PAT: Scale up and inline monitoring of protein refolding of Npro fusion proteins Process Biochemistry. ,vol. 49, pp. 1113- 1121 ,(2014) , 10.1016/J.PROCBIO.2014.03.022
Matthew P. DeLisa, Jincai Li, Govind Rao, William A. Weigand, William E. Bentley, Monitoring GFP‐operon fusion protein expression during high cell density cultivation of Escherichia coli using an on‐line optical sensor Biotechnology and Bioengineering. ,vol. 65, pp. 54- 64 ,(1999) , 10.1002/(SICI)1097-0290(19991005)65:1<54::AID-BIT7>3.0.CO;2-R
Cornelia Walther, Sabrina Mayer, Alexandru Trefilov, Gerhard Sekot, Rainer Hahn, Alois Jungbauer, Astrid Dürauer, Prediction of inclusion body solubilization from shaken to stirred reactors Biotechnology and Bioengineering. ,vol. 111, pp. 84- 94 ,(2014) , 10.1002/BIT.24998
Paul T. Wingfield, Ira Palmer, Shu‐Mei Liang, Folding and Purification of Insoluble (Inclusion-Body) Proteins from Escherichia coli Current protocols in protein science. ,vol. 78, pp. 651- 657 ,(1995) , 10.1002/0471140864.PS0605S00
Špela Peternel, Radovan Komel, Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells Microbial Cell Factories. ,vol. 9, pp. 66- 66 ,(2010) , 10.1186/1475-2859-9-66