Doping control analysis of bovine hemoglobin-based oxygen therapeutics in human plasma by LC-electrospray ionization-MS/MS.

摘要: Since January 2000, hemoglobin-based oxygen carriers, such as Hemopure, belong to the list of prohibited substances International Olympic Committee. Hemopure is based on bovine hemoglobin, which intra- and intermolecularly cross-linked by glutaraldehyde units causing an average molecular weight ∼250 000. Bovine human hemoglobins differ 15% in amino acid sequence; hence, tryptic digestion these proteins generates species-common -unique peptides. Those specific fragments originate from α- β-subunits Hb peptides α69-90 (2367.2 Da) or β40-58 (2089.9 Da). By means LC−MS/MS, hemoglobin can be separated identified, enabling determination compounds origin thus administration carriers Hemopure. Blank plasma samples were spiked with filtered, enzymatically digested, analyzed Agilent 1100 Series HPLC interfaced to...