Erythrocyte Amyloid Beta Peptide Isoform Distributions in Alzheimer and Mild Cognitive Impairment.

摘要: Introduction We recently showed that Amyloid Beta (Aβ)40 accumulates in erythrocytes and possibly causes cell damage as evidenced by an increased number of assumed injured low-density (kg/L) erythrocytes. Furthermore, we have suggested a separation technique to isolate concentrate such damaged red blood cells for subsequent analysis. Objectives isolated high- investigated the accumulation patterns Aβ peptides (Aβ40, Aβ42, Aβ43) Alzheimer (AD), mild cognitive impairment (MCI), Subjective Cognitive Impairment (SCI). Methods Whole was fractionated through density gradient, resulting two concentrated highand presumed erythrocyte fractions. After lysis, intracellular Aβ40, Aβ43 were quantified ELISA. Results In both erythrocytes, Aβ40 displayed lowest concentration MCI, while it equal higher AD SCI. detected at 10-fold level than quantity Aβ42 found MCI. exhibited 100-fold greater amount Aβ43, lighter MCI subjects less Conclusion Red differ significantly between AD, The data must be verified larger clinical trials. It is, however, tenable peptide distributions subpopulations potential used diagnostic purposes.