Detection of herpes simplex virus type 1 gene expression in latently and productively infected mouse ganglia using the polymerase chain reaction.

摘要: Summary The polymerase chain reaction (PCR) was employed to detect herpes simplex virus (HSV) sequences in the DNA, and HSV gene expression total cell RNA, extracted from cervical trigeminal ganglia of mice during productive latent infection with HSV-1, strain SC16. Such detected 1 µg or less quantity anticipated be present one two ganglia. Within limits primers available, latency appeared restricted latency-associated transcript (LAT). The 195 base portion LAT amplified by PCR sequenced found contain several changes deletions respect published for different strains. These mutations, within putative open reading frame 2 LAT, formed stop terminator signals, which suggests that does not act establish maintain through translation a protein. also 300 bp fragment any murine some other mammalian RNAs. Apart oligonucleotide primers, this did show homology HSV.