Serum low density lipoproteins with mitogenic effect on cultured aortic smooth muscle cells.

摘要: Low density lipoprotein (LDL) subspecies of different size and lipid mass were isolated by gradient ultracentrifugation from the serum male rhesus monkeys (Macaca mulatta) fed both a low fat, cholesterol commercial primate ration, cholesterol-supplemented high-fat diets, as well human donors. The mitogenic effect these lipoproteins was examined using primary cultures aortic smooth muscle cells. It observed that smaller LDL (molecular weight 2.7 × 106) normolipidemic small 2.6 occurring in some normal subjects exhibited no action. In turn, larger >3.0 106, buoyant <1.030 g/ml), whether or hyperlipidemic monkeys, subjects, had marked proliferative results indicate sera (both rhesus) contain species although amounts. LDL-III, equivalent Lp(a) not despite its similarity composition to stimulating particles. However, on removal most large sialic acid moiety, clear action observed. mechanisms responsible for are unclear may involve mass, composition, surface charge other speculations cannot at present be ruled out. Furthermore, since either origin nonmitogenic similar found calf serum, response cells LDLs depend their early conditioning with serum.