Real-Time Quantitative PCR of Telomere Length

摘要: Telomeres cap the ends of chromosomes and are essential for protection chromosomes, as well restricting replicative potential a cell. These functions achieved by regulation telomeric repeat length, making measurement telomere length useful aid in elucidation history cells. Previously published techniques employed either hybridization or flow cytometry methods, which technically demanding time-consuming. In 2002, R. M. Cawthon real-time polymerase chain reaction (PCR)-based method using Applied Biosystems Prism 7700 sequence detection system. The technique measures factor ratio copy number to single-gene differs between sample that reference deoxyribonucleic acid sample. many laboratories worldwide, including ours, PCR is carried out Roche LightCycler, opposed AB This benchmark details modifications Cawthon’s describes parameters reagents required measure LightCycler.