Mass spectrometry based metabolomics for Pseudomonas fluorescens : Investigating effects of anti-sigma factor MucA and carbon sources on metabolism

摘要: Metabolic profiling of Pseudomonas fluorescens SBW25 and various mutants derived thereof was performed to explore how the bacterium adapt changes in carbon source upon induction alginate synthesis. The experiments were at steady-state conditions nitrogen-limited chemostats using either fructose or glycerol as source. Carbon consumption up-regulated producing mutant with inactivated anti-sigma factor MucA. mucA- (also non-alginate control strains) had a higher dry weight yield on implying change energy metabolism due inactivation Both LC-MS/MS GC-MS methods used for quantitative metabolic profiling, major reorganization primary metabolite pools both an dependent manner observed. Generally, larger observed among phosphorylated glycolytic metabolites, pentose phosphate pathway metabolites nucleotide pool than amino acids citric acid cycle compounds. most significant observation level significantly reduced charge (both non-producing compared wild type strain. This reduction caused more by strong increase AMP ATP ADP pools. alginate-producing slightly increased GTP pool, while GDP GMP strongly strains type. Thus, whilst adenosine are attributed mucA inactivation, adjustments guanosine consequences GTP-dependent production induced inactivation. study provides new insight into producer P. fluorescens.