Effect of modification of plasma membrane fatty acid composition on fluidity and methotrexate transport in L1210 murine leukemia cells.

摘要: Abstract The plasma membrane lipid composition in L1210 murine leukemia cells was dependent upon the type of fat fed to host animal. Plasma membranes prepared from grown animals 16% sunflower oil contained 39% saturated, 14% monoenoic, and 41% polyenoic fatty acids. In contrast, those mice coconut 31% 23% Although there were 34% more unsaturated bonds on polyunsaturated fat, no difference phospholipid or cholesterol content membranes. Studies with spin label probes 5-nitroxide 12-nitroxide stearic acid indicated that order parameters at temperatures between 15 40° higher diet. transition temperature approximate rotational correlation time region 20° 2.5–3.5° isolated oil. These findings indicate fluidity altered by diet-induced modifications saturation. Appreciable differences noted when transport antineoplastic drug methotrexate intact diets compared. apparent V max not significantly different, K m for diet 2.90 ± 0.35 µm as compared 4.10 0.1 ( p