Neuropeptide Y is a neuromodulator of pulsatile luteinizing hormone-releasing hormone release in the gonadectomized rhesus monkey.

摘要: In a previous study, we have demonstrated that infusion of neuropeptide Y (NPY) into the stalk-median eminence (S-ME) gonadectomized rhesus monkeys stimulated LHRH in dose-dependent manner. This finding led us to address following questions: 1) What are characteristics NPY release vivo? 2) How does relate release? 3) Is endogenous essential pulsatile To answer these questions, three experiments using push-pull perfusion were performed adult monkeys. Perfusate samples from S-ME collected at 10-min intervals for 6 12-h periods, and concentrations perfusates determined by RIA. Exp I, pattern was independently group 11 conscious monkeys: with an interpulse interval 44.9 +/- 3.3 min (n = 11). similar seen (43.8 1.1 min, n 7). II designed determine whether pulses occur synchronously examine is correlated circulating LH pulses. aliquots same perfusate sample levels concurrently measured 8 sedated Saffan. It found temporally (P less than 0.001) pulses, which also Moreover, 0.05) peaks preceded 4.5 0.6 5.5 9.7 0.8 min. III, role evaluated infusing specific antiserum during Infusion 1:100 1:1000 dilutions suppressed significantly 0.05). nonimmune serum as control without effect. These results summarized follows: pulsatile, immunoneutralization suppresses release.(ABSTRACT TRUNCATED AT 400 WORDS)