QTL analysis of cadmium and zinc accumulation in the heavy metal hyperaccumulator Thlaspi caerulescens

摘要: Thlaspi caerulescens (Tc; 2n = 14) is a natural Zn, Cd and Ni hyperaccumulator species belonging to the Brassicaceae family. It shares 88% DNA identity in coding regions with Arabidopsis thaliana (At) (Rigola et al. 2006). Although physiology of heavy metal (hyper)accumulation has been intensively studied, molecular genetics are still largely unexplored. We address this topic by constructing genetic map based on AFLP® markers expressed sequence tags (ESTs). To establish map, an F2 population 129 individuals was generated from cross between plant Pb/Cd/Zn-contaminated site near La Calamine, Belgium, comparable Ganges (GA), France. These two accessions show different degrees Zn and, particularly, accumulation. analyzed 181 AFLP (of which 4 co-dominant) 13 co-dominant EST sequences-based mapped them seven linkage groups (LGs), presumably corresponding chromosomes T. caerulescens. The total length 496 cM average density one marker every 2.5 cM. This used for Quantitative Trait Locus (QTL) mapping F2. For as well concentration root we QTLs. Three QTLs QTL were shoot, respectively. explain 23.8–60.4% variance traits measured. found only common locus (LG6) (concentration root) shoot concentrations (LG1) (LG3). all QTLs, GA allele increased trait value except accumulation (LG1 LG4) (LG1).