Precise localization of aphidicolin-induced breakpoints on the short arm of human chromosome 3.

摘要: The common fragile site at 3p14.2 (FRA3B) has been described as the most active in human genome. This locus may predispose chromosome 3 to specific losses due deletions and translocations that have associated with several malignancies, including hereditary renal cell carcinoma. We previously induction of breakage around FRA3B using aphidicolin a somatic hybrid whose only component was single intact 3. That work led isolation hybrids breakpoints 3p13-p21.1 region loss all sequences distal their respective breakpoints. In this report we describe further characterization many these lines newly available molecular markers. also identification YAC clones span present hybrids.