Global transcriptome analysis of hexavalent chromium stress responses in Staphylococcus aureus LZ-01

摘要: Staphylococcus aureus strain LZ-01, isolated from the Lanzhou reaches of Yellow River, is capable reducing Cr(VI) to Cr(III) aerobically. We employed transcriptome sequencing analysis identify genes involved in stress responses S. LZ-01. Our results showed that 512 2,370 predicted displayed up-regulation (>2-fold), and 49 were down-regulated (<50 %) after exposure. Among up-regulated genes, 128 annotated encode proteins cellular processes; 68 categorized transport binding proteins; 26 DNA repair 32 associated with regulatory functions. To further elucidate resistance reduction mechanism, we carried out physiological tests quantitative PCR analysis. Both RNA-seq qRT-PCR data encoding a thioredoxin reductase main subunits cytochrome c oxidase complex upon treatment. Either cadmium or NaN3 treatment could inhibit which indicates are 29 ABC-type metal/multidrug transporters efflux pumps up-regulated, suggesting they by pumping chromium ions cells. The demonstrate toxic those protection need be responded for stress. Based on these results, mechanism LZ-01 resists reduces revealed.