13CO2/12CO2 exchange fluxes in a clamp‐on leaf cuvette: disentangling artefacts and flux components
作者： Xiao Ying Gong , Rudi Schäufele , Wolfgang Feneis , Hans Schnyder
DOI: 10.1111/PCE.12564
关键词:
摘要: Leaks and isotopic disequilibria represent potential errors artefacts during combined measurements of gas exchange carbon isotope discrimination (Δ). This paper presents new protocols to quantify, minimize, correct such phenomena. We performed experiments with gradients CO2 concentration (up ±250 μmol mol(-1) ) δ(13) CCO2 (34‰), between a clamp-on leaf cuvette (LI-6400) surrounding air, assess (1) leak coefficients for , (12) (13) the empty intact leaves Holcus lanatus (C3 or Sorghum bicolor (C4 in cuvette; (2) net photosynthesis dark respiration light. Leak were virtually identical but ∼8 times higher cuvette. generated on Δ up 6‰ H. lanatus 2‰ S. bicolor full light; produced similar variation Δ. darkness much larger due small biological : leak flux ratios. fully corrected determined same as measurements. Analysis enabled partitioning respiration, indicated inhibitions light (H. lanatus: 14%, S. bicolor: 58%).
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