Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development

摘要: The peripheral sensory nerves that innervate the cornea can be easily damaged by trauma, surgery, infection or diabetes. Several growth factors and axon guidance molecules, such as Semaphorin3A (Sema3A) are upregulated upon injury. Nerves regenerate after injury but do not recover their original density patterning. Sema3A is a well known cone repellent protein during development, however its role in adult nerve regeneration remains undetermined. Here we investigated neuro-regenerative potential of on nervous system neurons those cornea. First, examined gene expression profile Semaphorin class 3 family members found all expressed However, there fast increase expression. We then corroborated totally abolished promoting effect factor (NGF) embryonic observed signs collapse axonal retraction 30 min addition. isolated trigeminal ganglia dorsal root neurons, did inhibited NGF-induced neuronal growth. Furthermore, treated with alone produced similar to cells NGF length neurites branching was comparable between both treatments. These effects were replicated vivo, where thy1-YFP neurofluorescent mice subjected epithelium debridement receiving intrastromal pellet implantation containing showed increased corneal than pellets vehicle. In PNS potent inducer vitro vivo. Our data indicates functional switch for well-described repulsive development changes adulthood. high normal injured corneas could related factor.