Transferrin Promotion of 67Ga and 59Fe Uptake by Cultured Mouse Myeloma Cells

摘要: Radiotracer 67Ga-citrate is used as a tumor-seeking agent in clinical imaging investigations although fundamental reasons for its high uptake certain malignant lesions remain unexplained. The mechanism by which 67Ga becomes concentrated tumor cells has been investigated comparing and 59Fe cultured mouse myeloma with particular reference to stimulation transferrin. Concentrations of human transferrin down 2 microgram/ml greatly stimulated cellular both tracers, whereas bovine proved relatively inactive. rates tracers were similar was their degree retention cells, but quantitative dependencies on concentration showed characteristic differences. Pretreatment saturating amounts nonradioactive Fe3+ canceled ability promote uptake, it had little effect promotion uptake. Further increase the amount added did cause progressive depression this probably relates iron distribution whole-cell culture system including fetal calf serum component cell growth medium. results suggest that reveal different aspects interaction cells.