Antiviral activity of interferon against transmissible gastroenteritis virus in cell culture and ligated intestinal segments in neonatal pigs

摘要: Abstract Segments of jejunum in 5 to 6 days old piglets were surgically ligated, inoculated with transmissible gastroenteritis virus (TGEV) and 18 hours later the segments fixed for histology or suspensions prepared plaque assay swine testis (ST) cell cultures determine yield virus. When virulent Purdue strain TGEV was used, villous atrophy seen antigen demonstrated immunohistochemically enterocytes. The Miller M6 produced less extensive lesions segments, but since it titratable by used subsequent reduction assays antiviral activity interferon. intestinal simultaneously either 3200 units natural porcine interferon-α up 100000 recombinant human 2a , TGEV, there no reductions yield, although same cytokines exerted an effect ST cells treated a similar way. However, yields significantly reduced parenterally teh synthetic interferon inducer polyinosinic: polycytidylic acid before challenge TGEV. There also trend effects induction be augmented inclusion inoculum. It concluded that would ineffective as therapeutic might useful prophylactically.