High-performance liquid chromatography method for the characterization of rhamnolipid mixtures produced by pseudomonas aeruginosa UG2 on corn oil.
作者： Juan C Mata-Sandoval , Jeffrey Karns , Alba Torrents
DOI: 10.1016/S0021-9673(99)00979-6
关键词:
摘要: A HPLC method was developed to quantify rhamnolipid species in a bacterial biosurfactant mixture. The mixtures containing mainly 3-[3'-(L-rhamnopyranosyl-oxy)decanoyloxy]decanoic acid (RhC10C10), 3-[3'-(2'-O-alpha-L-rhamnopyranosyl-oxy)decanoyloxy]decanoic (Rh2C10C10), 3-[3'-(2'-O-alpha-L-rhamnopyranosyl-oxy)decanoyloxy]dodecanoic (Rh2C10C12), and dehydrogenated variety of the latter, 3-[3'-(2'-O-alpha-L-rhamnopyranosyl-oxy)decanoyloxy]dodecenoic (Rh2C10C12-H2), were isolated from Pseudomonas aeruginosa UG2 cultures grown on corn oil as sole carbon. identified quantified after their derivatization corresponding phenacyl esters. To confirm reliability method, further analyzed. Mass spectroscopy (electrospray ionization atmospheric pressure chemical techniques) used molecular mass, gas chromatography verify fatty content, colorimetric assay rhamnose content.
elsevier.com
sci-hub.se 参考文章(20)
David M. Falatko, John T. Novak, Effects of biologically produced surfactants on the mobility and biodegradation of petroleum hydrocarbons Water Environment Research. ,vol. 64, pp. 163- 169 ,(1992) , 10.2175/WER.64.2.10
Kosaric, Biosurfactants and Biotechnology ,(1987)
Y Zhang, R M Miller, Effect of a Pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegradation of octadecane. Applied and Environmental Microbiology. ,vol. 60, pp. 2101- 2106 ,(1994) , 10.1128/AEM.60.6.2101-2106.1994
Y Zhang, R M Miller, Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Applied and Environmental Microbiology. ,vol. 58, pp. 3276- 3282 ,(1992) , 10.1128/AEM.58.10.3276-3282.1992
A K Koch, O Käppeli, A Fiechter, J Reiser, Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. Journal of Bacteriology. ,vol. 173, pp. 4212- 4219 ,(1991) , 10.1128/JB.173.13.4212-4219.1991
P H Tomasek, J S Karns, Cloning of a carbofuran hydrolase gene from Achromobacter sp. strain WM111 and its expression in gram-negative bacteria. Journal of Bacteriology. ,vol. 171, pp. 4038- 4044 ,(1989) , 10.1128/JB.171.7.4038-4044.1989
Shahrukh A. Kanga, James S. Bonner, Cheryl A. Page, Marc A. Mills, Robin L. Autenrieth, Solubilization of Naphthalene and Methyl-Substituted Naphthalenes from Crude Oil Using Biosurfactants Environmental Science & Technology. ,vol. 31, pp. 556- 561 ,(1997) , 10.1021/ES9604370
Michele I. Dyke, Susan L. Gulley, Hung Lee, Jack T. Trevors, Evaluation of microbial surfactants for recovery of hydrophobic pollutants from soil Journal of Industrial Microbiology & Biotechnology. ,vol. 11, pp. 163- 170 ,(1993) , 10.1007/BF01583718
S. Arino, R. Marchal, J. -P. Vandecasteele, Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species Applied Microbiology and Biotechnology. ,vol. 45, pp. 162- 168 ,(1996) , 10.1007/S002530050665
LuisH. Guerra-Santos, Othmar K�ppeli, Armin Fiechter, Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors Applied Microbiology and Biotechnology. ,vol. 24, pp. 443- 448 ,(1986) , 10.1007/BF00250320