Transposome insertional mutagenesis and direct sequencing of microbial genomes.
作者： Les M. Hoffman , Jerry J. Jendrisak , Ronald J. Meis , Igor Y. Goryshin , William S. Reznikoff
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摘要: … Here we report the use of released complexes of Tn5 … represent the 19 base pair mosaic end (ME) sequences. R6Kγ … (2) insertion occurs by a standard Tn5 insertion mechanism. The …
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