A new search for thermotolerant yeasts, its characterization and optimization using response surface methodology for ethanol production.

摘要: The progressive rise in energy crisis followed by green house gas (GHG) emissions is serving as the driving force for bioethanol production from renewable resources. Current research focuses on lignocellulosic feedstocks these are abundantly available, renewable, sustainable and exhibit no competition between crops food fuel. However, technologies use have some drawbacks including incapability of pentose fermentation, reduced tolerance to products formed, costly processes, etc. Therefore, present study was carried out with objective isolating hexose fermenting thermophilic/thermotolerant ethanologens acceptable product yield. Two thermotolerant isolates, NIRE-K1 NIRE-K3 were screened both glucose xylose identified Kluyveromyces marxianus K. NIRE-K3. After optimization using Face-centered Central Composite Design (FCCD), growth parameters like temperature pH found be 45.17°C 5.49, respectively 45.41°C 5.24, Further, batch fermentations under optimized conditions, where superior over NIRE-K1. Ethanol yield (Y x∕s ), sugar ethanol conversion rate (%), microbial biomass concentration (X) volumetric productivity (Q p ) obtained 9.3, 9.55, 14.63, 31.94% higher than that NIRE-K1, respectively. This revealed promising potential isolates production.