PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins.
作者： Aitor Garzia , Pavel Morozov , Marcin Sajek , Cindy Meyer , Thomas Tuschl
DOI: 10.1007/978-1-4939-7540-2_5
关键词:
摘要: RNA-binding proteins (RBPs) establish posttranscriptional gene regulation (PTGR) by coordinating the maturation, editing, transport, stability, and translation of cellular RNAs. A variety experimental approaches have been developed to characterize RNAs associated with RBPs in vitro as well vivo. Our laboratory Photoactivatable-Ribonucleoside-Enhanced Cross-Linking Immunoprecipitation (PAR-CLIP), which combination next-generation sequencing enables identification RNA targets at a nucleotide-level resolution. Here we present an updated condensed step-by-step PAR-CLIP protocol followed description our RNA-seq data analysis pipeline.
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