Retrotransposon elements among initial sites of hepatitis B virus integration into human genome in the HepG2-NTCP cell infection model.

摘要: Abstract Integration of hepatitis B virus (HBV) DNA into host's genome is evident in all stages and models HBV infection. Investigations the initial virus-host junctions have been just recently initiated since their nature may promote liver oncogenesis immediately following We examined time-frame host sites at which integrates HepG2 cells overexpressing sodium taurocholate co-transporting polypeptide (NTCP) receptor mediating entry. HepG2-NTCP were analyzed from 15 min to 13 days post-infection (p.i.). The results showed that except for p.i., HBV-host integrations detected time points thereafter. At 30 min with retrotransposon SINE neuroblastoma breakpoint family member 1 gene detected. one-hour integration THE-1B-LTR was identified, while insertions proline-rich protein kinase cGMP-dependent type encoding genes found 3 h p.i. Fusion runt-related transcription factor 24 h merges 9 different day data elements are frequent among first-hit insertion. This suggest a mechanism by spread across earliest