Tissue Culture Models

摘要: The use of tissue cultures as a research tool to investigate the pathophysiological bases diseases has become essential in current ag molecular biomedical research. Although it will always be necessary translate and validate observations seen vitro patient or animal, ability role(s) individual variables free from confounders is paramount toward increasing our understanding physiology their role disease. Additionally, not feasible conduct certain humans because ethical constraints, yet investigators may still interested physiological response human tissues; characterization an acceptable choice. Tissue culture techniques have been utilized extensively questions pertaining isolation propagation epithelial cells allowed begin characterize interactions reactions that occur various stimuli. Moreover, other researchers pathological cascades well responses. Combining cultured endothelial leukocytes together under laminar flow conditions helped elucidate critical rolling emigration during inflammatory response. Cultured embryonic stem had subjected growth advanced cellular differentiation growth.