IgE from latex-allergic patients binds to cloned and expressed B cell epitopes of prohevein.

摘要: Prohevein is one of the major allergens associated with latex allergy. In present study, we identified IgE binding regions prohevein, and expressed multiple epitopes by selective cloning. These truncated polypeptides were then used to demonstrate in sera patients. Decapeptides prohevein synthesized on derivatized cellulose membrane an offset amino acid. The reactivity these linear peptides was evaluated separately using pooled from latex-allergic health care workers (HCW) spina bifida (SB) A total 10 representing unique as well shared both N- C-domains identified. Recombinant constructed based epitopes, clones carrying DNA fragments overexpressed. recombinant for HCW, SB, normal individuals. hevein, C-domain exhibited 84, 88, 40% HCW sera, respectively, against 84% crude allergens. However, only 48% SB patients showed while 56 28% had C-domains, respectively. Among three remaining C-domain, CA44-103 results study suggest that epitope analysis construction increase sensitivity specificity immunodiagnosis allergy provide more information immunopathogenesis hypersensitivity reaction mediated type I