Multiplex PCR: Optimization guidelines

作者: G. Zangenberg , R.K. Saiki , R. Reynolds

DOI: 10.1016/B978-012372185-3/50007-9

关键词:

摘要: … of interest simultaneously in a "multiplex" reaction. This chapter presents the optimization guidelines for multiplex PCR. There are various advantages of using multiplex PCR. It offers a …

参考文章(16)
Jeffrey S. Chamberlain, Joel R. Chamberlain, Optimization of Multiplex PCRs The Polymerase Chain Reaction. pp. 38- 46 ,(1994) , 10.1007/978-1-4612-0257-8_3
Chenchik A, Siebert Pd, Kellogg De, Chen S, Rybalkin I, Mukhamedova N, Vlasik T, TaqStart antibody : hot start PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase BioTechniques. ,vol. 16, pp. 1134- 1137 ,(1994)
Bruce Budowle, Jenifer A. Lindsey, Jacqueline A. DeCou, Barbara W. Koons, Alan M. Giusti, Catherine T. Comey, Validation and Population Studies of the Loci LDLR, GYPA, HBGG, D7S8, and Gc (PM loci), and HLA-DQα Using a Multiplex Amplification and Typing Procedure Journal of Forensic Sciences. ,vol. 40, pp. 45- 54 ,(1995) , 10.1520/JFS13758J
Mary C. Longo, Mark S. Berninger, James L. Hartley, Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions Gene. ,vol. 93, pp. 125- 128 ,(1990) , 10.1016/0378-1119(90)90145-H
Quin Chou, Marion Russell, David E. Birch, Jonathan Raymond, Will Bloch, Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Research. ,vol. 20, pp. 1717- 1723 ,(1992) , 10.1093/NAR/20.7.1717
David E. Birch, L. Kolmodin, J. Wong, G. A. Zangenberg, M. A. Zoccoli, N. McKinney, K. K. Y. Young, Simplified hot start PCR Nature. ,vol. 381, pp. 445- 446 ,(1996) , 10.1038/381445A0
Colin Kimpton, Deborah Fisher, Stephanie Watson, Maia Adams, Andy Urquhart, Joan Lygo, Peter Gill, Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci International Journal of Legal Medicine. ,vol. 106, pp. 302- 311 ,(1994) , 10.1007/BF01224776
R. Saiki, D. Gelfand, S Stoffel, S. Scharf, R Higuchi, G. Horn, K. Mullis, H. Erlich, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Science. ,vol. 239, pp. 487- 491 ,(1988) , 10.1126/SCIENCE.2448875
Jeffrey S. Chamberlain, Richard A. Gibbs, Joel E. Rainer, Phi Nga Nguyen, C. Thomas, Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification Nucleic Acids Research. ,vol. 16, pp. 11141- 11156 ,(1988) , 10.1093/NAR/16.23.11141
O. Henegariu, N.A. Heerema, S.R. Dlouhy, G.H. Vance, P.H. Vogt, Multiplex PCR: critical parameters and step-by-step protocol. BioTechniques. ,vol. 23, pp. 504- 511 ,(1997) , 10.2144/97233RR01