Altered fungal sensitivity to a plant antimicrobial peptide through over-expression of yeast cDNAs.
作者： Camilla Stephens , Stuart J. Harrison , Kemal Kazan , Frank W. N. Smith , Ken C. Goulter
DOI: 10.1007/S00294-005-0562-8
关键词:
摘要: A yeast cDNA expression library was screened to identify genes and cellular processes that influence fungal sensitivity a plant antimicrobial peptide. plasmid-based, GAL1 promoter-driven introduced into genotype susceptible the peptide MiAMP1 purified from Macadamia integrifolia. Following screen of 20,000 cDNAs, three cDNAs were identified reproducibly provided transformants with galactose-dependent resistance MiAMP1. These encoded protein unknown function, component (VMA11) vacuolar H+-ATPase (cytochrome c oxidase subunit VIa) mitochondrial electron transport chain, respectively. To increased MiAMP1, mutant From 11,000 screened, two clones corresponding ser/thr kinase phosphatase susceptibility in manner. Deletion mutants available for five but showed no change their indicating these could not be detected by screening deletion libraries. Yeast therefore provides an alternative approach gene libraries can fungi peptides.
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