New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535.

摘要: A new method for gene disruption in Salmonella typhimurium was developed. The key steps of this are to produce restriction fragments with compatible ends, preligate concatemers, and then transform by electrotransformation. We developed used construct a mutant S. TA1535 which the resident ada-like (adaST) replaced kanamycin resistance an adaST-deletion derivative. strain did not exhibit higher level mutability upon treatment N-methyl-N'-nitro-N-nitrosoguanidine than its wild-type parent strain. However, it sensitivity respect killing N-methyl-N'-nitro-N-nitrosoguanidine. ability AdaST function as transcriptional activator is discussed.