作者: Jing Wang , Jinjun Kan , Laura Borecki , Xiaodong Zhang , Dongxiao Wang
DOI: 10.1007/S13131-016-0871-4
关键词:
摘要: Besides being critical components of marine food web, microorganisms play vital roles in biogeochemical cycling nutrients and elements the ocean. Currently little is known about microbial population structure their distributions eastern Indian Ocean. In this study, we applied molecular approaches including polymerase chain reaction-denaturant gradient gel electrophoresis (PCR-DGGE) High-Throughput next generation sequencing to investigate bacterial 16S rRNA genes from equatorial regions adjacent Bay Bengal general, Bacteroidetes, Proteobacteria (mainly Alpha, Gamma), Actinobacteria, Cyanobacteria Planctomycetes dominated communities. Horizontally distinct spatial distribution major groups was observed PCR-DGGE image analyses. However, further detailed characterization community structures by pyrosequencing suggested a more pronounced stratified pattern: Actinobacteria were predominant at surface water (25 m); Bacteroidetes 25 m 150 while Alphaproteobacteria) occurred frequently 75 depth. With increasing depth, communities different locations tended share high similarity, indicating niche partitioning for minor bacteria recovered with throughput approaches. This study provided first “snapshot” on biodiversity Bacteria columns Ocean, findings emphasized potential functional these microbes energy resource