Characteristics of French methicillin-resistant Staphylococcus aureus isolates with decreased susceptibility or resistance to glycopeptides

摘要: According to the French Society of Microbiology, Staphylococcus aureus isolates are suspected have decreased susceptibility glycopeptide(s) when at least one colony is able grow from an inoculum 10 microL 2 McFarland bacterial suspension plated on Mueller-Hinton agar containing 5 mg/L teicoplanin and incubated for 48 h 35-37 degrees C. We analysed 89 methicillin-resistant S. (MRSA), collected in 2000-2001 24 hospitals located 18 cities, which were this selective medium. These distributed into six groups basis their glycopeptide resistance phenotypes: (A) susceptible (GSSA, 21 isolates); (B) heterogeneous intermediately resistant (hetero-TISA, (C) both glycopeptides, vancomycin (hetero-GISA, (D) resistant/teicoplanin (hetero-VISA/TISA, 30 (E) GISA (four (F) TISA isolates). Despite persistent decrease gentamicin-resistant MRSA since 1993, prevalence very high D, E F. Moreover, most group C, D exhibiting glycopeptides belong same major SmaI genotype, has been detected Europe 1989.