Assay method and assay reagent for serum iron or unsaturated iron binding capacity

作者: Masatsugu Nonobe , Tsuyoshi Fujita , Hozumi Nishida

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摘要: The present invention measures serum iron by liberating from transferrin under acid conditions, and contacting a test sample with reaction system which utilizes at least aconitase, reducing reagent, citric acid, isocitrate dehydrogenase (thio)NAD(P). According to the invention, an accurate efficient measurement of in is possible, even for minute levels iron. In addition, assays unsaturated binding capacity adding excess bind transferrin, remaining unbound measuring activated aconitase. quick easy assay body fluids, etc. possible without need expert skill.

参考文章(14)
John M. Lowenstein, Citric acid cycle Academic Press. ,(1969)
Irwin A. Rose, Edward L. O'Connell, Mechanism of Aconitase Action I. THE HYDROGEN TRANSFER REACTION Journal of Biological Chemistry. ,vol. 242, pp. 1870- 1879 ,(1967) , 10.1016/S0021-9258(18)96082-5
Michael Nathaniel Prof. Berry, Michael-Harold Dr.Phil. Town, Uwe Dipl.Biochem.Dr.Rer.Nat. Herrmann, Georg-Burkhard Dipl.Chem.Dr.Rer.Nat. Kresse, Determination of potassium ions in fluids ,(1988)
Joseph J. Villafranca, Albert S. Mildvan, The mechanism of aconitase action. II. Magnetic resonance studies of the complexes of enzyme, manganese(II), iron(II), and substrates. Journal of Biological Chemistry. ,vol. 246, pp. 5791- 5798 ,(1971) , 10.1016/S0021-9258(18)61874-5
Susumu Takano, Yoshikey Yoneda, Determination of unsaturated iron-binding capacity ,(1984)
Henry G Traynor, Judith R Shiley, John A O'malley, Anne E Hassan, Method for the determination of serum iron and total iron binding capacity ,(1969)
Tracey A. Rouault, C.David Stout, Stamatina Kaptain, Joe B. Harford, Richard D. Klausner, Structural relationship between an iron-regulated RNA-binding protein (IRE-BP) and aconitase: Functional implications Cell. ,vol. 64, pp. 881- 883 ,(1991) , 10.1016/0092-8674(91)90312-M