Salt Modulates Bacterial Hydrophobicity and Charge Properties Influencing Adhesion of Pseudomonas Aeruginosa (PA01) in Aqueous Suspensions

作者: Jacob J. Shephard , David M. Savory , Phil J. Bremer , A. James McQuillan

DOI: 10.1021/LA1007878

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摘要: The influence on cell hydrophobicity of differential extension with ionic strength lipopolysaccharide molecules (LPS), which exist as charged and uncharged polymers at the surface Gram-negative bacterium Pseudomonas aeruginosa (PA01), has been investigated. Attenuated total reflection infrared (ATR-IR) spectral absorptions from a single layer cells adsorbed to ZnSe increased in intensity increasing NaCl concentration up 0.1 mol L−1. Dynamic contact angle measurements (Wilhelmy plate tensiometry) made having an adherence hexadecane suggest that PA01 were most hydrophobic L−1 solutions. These data indicate charge screening induced compression LPS decreasing cell-surface approach distance due greater predominance polymers. Interestingly, layers 0.3 had lower...

参考文章(39)
Henry J. Vogel, David M. Bonner, Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry. ,vol. 218, pp. 97- 106 ,(1956) , 10.1016/S0021-9258(18)65874-0
M Rivera, L E Bryan, R E Hancock, E J McGroarty, Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length. Journal of Bacteriology. ,vol. 170, pp. 512- 521 ,(1988) , 10.1128/JB.170.2.512-521.1988
S. Bayoudh, A. Othmane, F. Bettaieb, A. Bakhrouf, H. Ben Ouada, L. Ponsonnet, Quantification of the adhesion free energy between bacteria and hydrophobic and hydrophilic substrata Materials Science and Engineering: C. ,vol. 26, pp. 300- 305 ,(2006) , 10.1016/J.MSEC.2005.10.045
Yongsuk Hong, Derick G. Brown, Electrostatic behavior of the charge-regulated bacterial cell surface. Langmuir. ,vol. 24, pp. 5003- 5009 ,(2008) , 10.1021/LA703564Q
J. B. Smathers, E. Kneedler, B. R. Bennett, B. T. Jonker, Nanometer scale surface clustering on ZnSe epilayers Applied Physics Letters. ,vol. 72, pp. 1238- 1240 ,(1998) , 10.1063/1.121025
Ritsu Sonohara, Nobuhiro Muramatsu, Hiroyuki Ohshima, Tamotsu Kondo, Difference in surface properties between Escherichia coli and Staphylococcus aureus as revealed by electrophoretic mobility measurements Biophysical Chemistry. ,vol. 55, pp. 273- 277 ,(1995) , 10.1016/0301-4622(95)00004-H
Nily Dan, The effect of charge regulation on cell adhesion to substrates: salt-induced repulsion Colloids and Surfaces B: Biointerfaces. ,vol. 27, pp. 41- 47 ,(2003) , 10.1016/S0927-7765(02)00041-3