The novel virulence-related gene nlxA in the lipopolysaccharide cluster of Xanthomonas citri ssp. citri is involved in the production of lipopolysaccharide and extracellular polysaccharide, motility, biofilm formation and stress resistance
作者： QING YAN , XIUFANG HU , NIAN WANG
DOI: 10.1111/J.1364-3703.2012.00800.X
关键词:
摘要: SUMMARY Lipopolysaccharide (LPS) is an important virulence factor of Xanthomonas citri ssp. citri, the causative agent citrus canker disease. In this research, a novel gene, designated as nlxA (novel LPS cluster gene X. citri citri), in 306, was characterized. Our results indicate that required for O-polysaccharide biosynthesis by encoding putative rhamnosyltransferase. This supported several lines evidence: (i) NlxA shares 40.14% identity with WsaF, which acts rhamnosyltransferase; (ii) sodium dodecylsulphate-polyacrylamide gel electrophoresis analysis showed four bands O-antigen part were missing production mutant; also consistent previous report moiety composed rhamnose homo-oligosaccharide; (iii) mutation resulted significant reduction resistance to different stresses, including dodecylsulphate, polymyxin B, H2O2, phenol, CuSO4 and ZnSO4. addition, our plays role extracellular polysaccharide production, biofilm formation, stress resistance, motility on semi-solid plates, in planta growth host plant grapefruit.
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