Methylation of S100A8 is a promising diagnosis and prognostic marker in hepatocellular carcinoma

摘要: // Kun Liu 1, 5, * , Yuening Zhang Chengdong 6, Qinle 2, Jiatong Li Feifan Xiao 1 Yingfang 3 Ruoheng Dongwei Dou Jiezhen Liang 4 Jian Qin 7 Zhidi Lin Dong Zhao Min Jiang Zhenxin Jie Su 8 Vanaparthy Pranay Gupta He Xiaoli Yang Medical Scientific Research Center, Guangxi University, Nanning, 530021, China 2 Genetic and Metabolic Central Laboratory, The Maternal Children Health Hospital of Guangxi, 530002, Department Endocrinology, Fourth Affiliated Liuzhou, 545005, Otolaryngology-Head Neck Surgery, First 5 Gastroenterology, Third Nanchang City, Jiangxi 330009, 6 Microbiology Microbial Engineering, School Life Sciences, Fudan Shanghai 200438, Public Health, Key Laboratory Early Prevention Treatment for Regional High Frequency, Ministry Education, These authors have contributed equally to this work Correspondence to: Yang, email: cncsyxl@126.com He, hemin@gxmu.edu.cn Keywords: hepatocellular carcinoma, S100A8, DNA methylation, pyrosequencing, prognosis Received: October 03, 2015     Accepted: June 29, 2016     Published: July 23, 2016 ABSTRACT abnormality methylation is one the major epigenetic alterations in human carcinoma (HCC). We assessed global genomic profiles HCC patients by using Infinium Human Methylation27 BeadChip. A CpG loci S100A8 was found be significantly hypomethylated HCC. Pooled meta-analysis five validation public datasets demonstrated its level lower compared paired adjacent normal tissues. Quantitative pyrosequencing analysis also showed that decreased cancer tissues (31.90%±13.31%) than (65.33%±3.64%, p <0.01). area under ROC curve (AUC) value 0.950 ( Kaplan-Meier survival curves revealed hypomethylation associated with shortened overall (OS) progression-free (PFS) (log rank <0.05). Multivariate Cox proportional hazards model indicated shorter OS (HR, 1.709; 95 % CI, 1.127–2.591) PFS 1.767; 1.168–2.974) were observed low-methylation-level group high-methylation-level group. Furthermore, overexpression Huh7 MHCC-97H hepatoma cell lines led increased proliferation, migration, invasion, tumor growth. findings suggested served as potential diagnosis marker may play a promoter