An in vitro 3D diabetic human skin model from diabetic primary cells.

摘要: Diabetes mellitus, a complex metabolic disorder, leads to many health complications like kidney failure, diabetic heart disease, stroke, and foot ulcers. Treatment approaches of diabetes identification the mechanisms underlying skin have gained importance due continued rapid increase in incidence. A thick pre-vascularized vitro 3D type 2 human model (DHSM) was developed this study. The methacrylated gelatin (GelMA) hydrogel produced by photocrosslinking its pore size (54.85 ± 8.58 μm), compressive modulus (4.53 0.67 kPa) swelling ratio (17.5 2.2%) were found be suitable for tissue engineering. 8% GelMA effectively supported viability, spreading proliferation dermal fibroblasts. By isolating fibroblasts, umbilical vein endothelial cells keratinocytes from patients, an DHSM, 12 mm width 1.86 thick, constructed. consisted continuous basal epidermal layer with blood capillary-like structures, ideal evaluating effects anti-diabetic drugs wound healing materials factors. functionality DHSM showed applying therapeutic into central wound; especially fibroblast migration site evident 9 d. We demonstrated that is biologically relevant sensitivity predictability potential material.