Biosynthesis of the acetyl-CoA carboxylase-inhibiting antibiotic, andrimid in Serratia is regulated by Hfq and the LysR-type transcriptional regulator, AdmX.

摘要: Infections due to multidrug-resistant bacteria represent a major global health challenge. To combat this problem, new antibiotics are urgently needed and some plant-associated promising source. The rhizobacterium Serratia plymuthica A153 produces several bioactive secondary metabolites, including the anti-oomycete antifungal haterumalide, oocydin A broad spectrum polyamine antibiotic, zeamine. In study, we show that second andrimid. Using genome sequencing, comparative genomics mutagenesis, defined genes involved in andrimid (adm) biosynthesis. Both expression of adm gene cluster regulation synthesis were investigated. biosynthetic is operonic its modulated by various environmental cues, temperature carbon Analysis context operon revealed encoding predicted LysR-type regulator, AdmX, apparently unique strains. Mutagenesis assays demonstrated AdmX transcriptional activator cluster. At post-transcriptional level, positively regulated RNA chaperone, Hfq, an RpoS-independent manner. Our results highlight complexity biosynthesis - antibiotic with potential clinical agricultural utility.