Studies on a promising anticancer molecule of marine origin: results of an interdisciplinary study

摘要: Poly-APS, a mixture of two 3-octylpyridinium polymers, including 29 and 99 monomeric units, extracted from the Mediterranean sponge, Haliclona (Reniera) sarai, was demonstrated to exert strong specific non-toxic acetylcholinesterase inhibition in vitro. Since first '80s, we found that some tumour types, particular lung tumours present overexpression activity. Acetylcholinesterase is an enzyme associated tothe cholinergic signal system, but also involved cell-tocell communication driving embryonic development regulation several cellular features, such as apoptosis cell movements, cells biopsies. Cytotoxicity tests on immortalized primarycell lines derived (NSCLC) showed poly-APS dose-dependent selective reduction viability, statistically significant. The same cells, exposed salts exhibited loss inthe mitochondrial potential, positive response annexin V assay, T-terminal are markers apoptoticevent. What makes promising anticancer therapy adjuvant they, at concentrations inducing do not affect viability lymphocytes isolated healthy patients. Moreover, three-dimensional cultures (spheroids) exposure show decrease membrane-linked oligosaccharides, responsible for adhesivity metastatic cells. no effects were organs, heart, liver, kidney mice treated by poly-APS, vivo amass density significantly reduced. In this frame, theneed emerges isolation synthetic homologs molecules, order start study therapeutical application drug.