Expression cloning of cholesterol α-glucosyltransferase, a unique enzyme that can be inhibited by natural antibiotic gastric mucin O -glycans, from Helicobacter pylori
作者： Heeseob Lee , Motohiro Kobayashi , Ping Wang , Jun Nakayama , Peter H. Seeberger
DOI: 10.1016/J.BBRC.2006.08.145
关键词:
摘要: Helicobacter pylori infects over half the world's population, but only 3% of those infected develop peptic ulcer, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. In H. pylori, alpha-glucosyl cholesterol constitutes more than 25% cell wall lipids, it has been suggested that is essential for viability. Here, we identified alpha-glucosyltransferase (CHLalphaGcT) using an expression cloning strategy showed this enzyme distinctively inhibited by mucin-type O-glycans similar to present in deeper portions mucosa. Moreover, inactivation CHLalphaGcT homologous recombination led lethality. These results indicate a unique targeted natural antibiotic mucin excellent therapeutic target prevent pylori-induced carcinoma, MALT
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