Induction of iron-derived EPR signals in murine cancers by nitric oxide. Evidence for multiple intracellular targets.
作者： N.R. Bastian , C.Y. Yim , J.B. Hibbs , W.E. Samlowski
DOI: 10.1016/S0021-9258(17)37664-0
关键词:
摘要: The cell-mediated immune response to syngeneic tumors activates the cytokine-inducible nitric oxide synthase. We observed that murine exhibited EPR signals related iron-nitrosyl complex formation. Three different active species were observed, an Fe(RS)2(NO)2 signal and two differentiable heme-nitrosyl complexes. Hemoglobin assays showed not derived from contaminating hemoglobin. Signal amplitudes attenuated in mice treated with N omega-mono-methyl-L-arginine (MLA), inhibitor of Tumors grown vivo contained while those culture without continuing cytokine stimulation lost after a few days. Cultured cells cytokines, or cocultivated cytokine-activated macrophages, regained These results show involves induction While synthesis is induced both tumor infiltrating macrophages themselves, only contributed formation This result indicates presence novel intracellular target for NO within cells.
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