Studies on the lipid composition of the rat liver endoplasmic reticulum after induction with phenobarbitone and 20-methylcholanthrene.

摘要: Abstract 1. The cholesterol content, proportions of different phospholipids and fatty acid components phosphatidylcholine phosphatidylethanolamine were studied in rat liver endoplasmic-reticulum membrane, after a single injection 20-methylcholanthrene or injections phenobarbitone for 5 days. 2. A marked decrease the proportion occurred days phenobarbitone. 3. was increased by minor changes other phospholipids. 4. Phenobarbitone caused linoleic to increase 120–125% control oleic acid, arachidonic docosahexaenoic decrease. 5. 20-Methylcholanthrene an ethanolamine 125–140% control, 1 day injection. 6. occurs simultaneously with cytochrome P-450 concentration, rate oxidative demethylation aminopyrine hydroxylation biphenyl. It is therefore considered that distinct species containing β position are essential membrane optimal activity demethylation.