Interaction of molluscs and foreign substances: The morphology and behavior of hemolymph cells of the American oyster, Crassostrea virginica, in vitro

摘要: Hemolymph cells of Crassostrea virginica have been studied in the living state with apochromatic and brightfield-phase contrast microscopy fixed stained brightfield Nomarski optics. Cells two size populations recognized. The large are classes: granulocytes fibrocytes. Granulocytes commonly include mixtures acidophilic, basophilic, refractile granules believed to be one general type. Fibrocytes subcategorized as being primary secondary types, first enclosing lobate nuclei second spherical or ovoid nuclei. small cells, designated hyalinocytes, either agranular slightly granular. The behavior each type cell vitro is described. cytoplasm becomes distinctly separated into ectoplasm granular endoplasm upon standing; furthermore, semirigid “rays” extend from periphery. Ectoplasmic fans connect “rays”. characteristically produce elongate pseudopods. Hyalinocytes capable producing filopods lobopods but do not spread like granulocytes. With characterization types present C. hemolymph, it now possible study constituents roles their reactions foreign substances.