Identification of a new glycoprotein of herpes simplex virus type 1 and genetic mapping of the gene that codes for it.

摘要: A type-specific monoclonal antibody, LP10, precipitated a glycoprotein with molecular weight of approximately 59,000 from purified herpes simplex virus type 1. Although this was similar in size to D (gD), it shown be less abundant both virions and infected cells, migrate more rapidly its precursor form, incorporate glucosamine but not mannose, have stable tunicamycin-treated cells than the gD (pgD). Immunoassays insertion recombinants intertypic localized gene coding for target antigen LP10 unique short (Us) region at map units 0.892 0.924 excluding gD. The then definitively mapped Us4 open reading frame by immunoprecipitation polypeptide synthesized vitro translation Us4-specific transcript prepared using an SP6 cloning This newly identified product 1 is distinct previously gB1, gC1, gE1, gH1.