X-ray repair cross-complementing protein 1 associated multiprotein complexes in base excision repair

摘要: XRCC1 functions as a non-enzymatic, scaffold protein in single strand break repair (SSBR) and base excision (BER). Here, we examine different regions of for their contribution to the scaffolding protein. We found that central BRCT1 domain is essential recruitment sites DNA damage replication. Also, ectopic expression region from residue 166-436 partially rescued methyl methanesulfonate (MMS) hypersensitivity XRCC1-deficient EM9 cells, suggesting key role this mediating repair. The three most common amino acid variants XRCC1, Argl 94Trp, Arg280His Arg399G1n, are located within comprising NLS domains, these may be associated with increased incidence specific types cancer. While could not detect differences intra-nuclear localization or ability support POL beta PNKP micro-irradiated relative conservative protein, did observe lower foci intensity after micro-irradiation reduced stability Arg399GIn variants, respectively. Furthermore, when challenged MMS hydrogen peroxide, detected small but consistent profiles cells expressing two comparison (C) 2012 Elsevier B.V. All rights reserved.