Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit treesfn1fn1The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the following accession numbers: AB011827, Japanese pear (Pyrus pyrifolia cv. Housui); AB011828, pear (Pyrus communis cv. la france); AB011829, peach (Prunus persica); AB011830, Japanese loquat (Eriobotrya japonica); AB011831, Chinese quince (Pseudocydonia sinensis).
作者： Miyoshi Haruta , Masatsune Murata , Hiroshi Kadokura , Seiichi Homma
DOI: 10.1016/S0031-9422(98)00633-5
关键词:
摘要: An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified characterized. The deduced protein sequences showed identities 85.3 to 97.5%. Chlorogenic acid activity these higher activities when assayed at pH 4 than 6. These results indicate that in Rosaceae plants are structurally enzymatically similar.
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