Antifungal activity of synthetic peptide derived from halocidin, antimicrobial peptide from the tunicate,Halocynthia aurantium

摘要: Halocidin is an antimicrobial peptide isolated from the hemocytes of tunicate. Among several known synthetic halocidin analogues, di-K19Hc has been previously confirmed to have most profound antibacterial activity against antibiotic-resistant bacteria. This considered be effective candidate for development a new type antibiotic. In this study, we assessed antifungal di-K19Hc, panel fungi including strains Aspergillus and Candida. As result, determined that MICs six Candida albicans two species were below 4 16 microg/ml, respectively, thereby indicating may appropriate treatment fungal diseases. We also conducted investigation into di-K19Hc's mode action albicans. Our colony count assay showed killed C. within 30s. Di-K19Hc bound surface via specific interaction with beta-1,3-glucan, which one cell wall components. induced formation ion channels membrane albicans, eventually observed death, was measurements K+ released cells had treated as well by monitoring uptake propidium iodide cells. membrane-attacking quality visualized confocal laser scanning electron microscopy.